Indirect Antiglobulin (Indirect Coombs) Test
The indirect antiglobulin (indirect Coombs) test is used to detect IgG antibodies against RBCs in a patient's plasma. The patient's serum or plasma is incubated with reagent RBCs; then Coombs serum (antibodies to human IgG, or human anti-IgG) is added. If agglutination occurs, IgG antibodies (autoantibodies or alloantibodies) against RBCs are present. This test is also used to determine the specificity of an alloantibody.
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