Thalassemias

(Mediterranean Anemia; Thalassemia Major and Minor)

ByGloria F. Gerber, MD, Johns Hopkins School of Medicine, Division of Hematology
Reviewed/Revised Apr 2024
View Patient Education

Thalassemias are a group of inherited microcytic, hemolytic anemias characterized by defective hemoglobin synthesis. Alpha-thalassemia is particularly common among people with African, Mediterranean, or Southeast Asian ancestry. Beta-thalassemia is more common among people with Mediterranean, Middle Eastern, Southeast Asian, or Indian ancestry. Symptoms and signs result from anemia, hemolysis, splenomegaly, bone marrow hyperplasia, and, if there have been multiple transfusions, iron overload. Diagnosis is based on genetic tests and quantitative hemoglobin analysis. Treatment for severe forms may include transfusion, splenectomy, chelation, and stem cell transplantation.

(See also Overview of Hemolytic Anemia.)

Pathophysiology of Thalassemias

Thalassemia is a hemoglobinopathy that is among the most common inherited disorders of hemoglobin production. The normal adult hemoglobin molecule (Hb A) consists of 2 pairs of chains designated alpha and beta. Normal adult blood also contains 2.5% Hb A2 (composed of alpha and delta chains) and < 1.4% hemoglobin F (fetal hemoglobin), which has gamma chains in the place of beta chains. Thalassemia results from unbalanced hemoglobin synthesis caused by decreased production of at least one globin polypeptide chain (beta, alpha, gamma, delta).

Alpha-thalassemia

Alpha-thalassemia results from decreased production of alpha-polypeptide chains due to a deletion of one or more alpha genes. People normally have 4 alpha genes (2 on each of a pair of chromosomes) because the alpha gene is duplicated. Disease classification is based on the number and location of deletions:

  • Alpha + thalassemia: Loss of a single gene on 1 chromosome (alpha/--)

  • Alpha 0 thalassemia: Loss of both genes on the same chromosome (--/--)

Beta-thalassemia

Beta-thalassemia results from decreased production of beta-polypeptide chains due to either mutations or deletions in the beta globin gene, leading to impaired production of hemoglobin (Hb) A. Mutations or deletions may result in partial loss (beta + allele) or complete loss (beta 0 allele) of beta globin function. There are 2 beta globin genes, and patients may have heterozygous, homozygous, or compound heterozygous mutations.

In addition, patients may be heterozygous or homozygous for abnormalities in 2 different globin genes (eg, beta and delta).

Beta-delta-thalassemia is a less common form of beta-thalassemia in which production of both the delta chain as well as the beta chain is impaired. These mutations may be heterozygous or homozygous.

Symptoms and Signs of Thalassemias

Clinical features of thalassemias are similar but vary in severity depending on the amount of normal hemoglobin present.

Alpha-thalassemia

Patients with a single alpha + allele (alpha/alpha;alpha/--) are clinically normal and are called silent carriers.

Patients who are heterozygous with defects in 2 of the 4 genes such as two alpha + alleles (alpha/--;alpha/--) or one alpha 0 allele (alpha/alpha;--/--) tend to develop mild to moderate microcytic anemia but no symptoms. These patients have alpha-thalassemia trait (also called alpha thalassemia minor).

Defects in 3 of the 4 genes caused by coinheritance of both alpha + and alpha 0 (alpha/--;--/--) severely impair alpha-chain production. Impaired alpha-chain production results in the formation of tetramers of excess beta-chains termed Hb H or, in infancy, gamma-chains termed Bart’s hemoglobin. Patients with Hb H disease often have symptomatic hemolytic anemia and splenomegaly.

Defects in all 4 genes via 2 alpha 0 alleles (--/--;--/--) is a lethal condition in utero (hydrops fetalis), because hemoglobin that lacks alpha chains cannot transport oxygen.

Beta-thalassemia

In beta-thalassemia, clinical phenotypes are classified into 3 groups based on the degree to which beta globin production is impaired:

  • Minor (or trait)

  • Intermedia

  • Major

Beta-thalassemia minor (trait) occurs in patients who are heterozygous (beta/beta + or beta/beta 0). These patients are usually asymptomatic with mild to moderate microcytic anemia. This phenotype may also occur in mild cases of beta +/beta +.

Beta-thalassemia intermedia is a variable clinical picture that is intermediate between thalassemia major or minor, caused by inheritance of 2 beta thalassemia alleles (beta +/beta 0 or severe cases of beta +/beta +).

Beta-thalassemia major (or Cooley anemia) occurs in patients who are homozygous (beta 0/beta 0) or severe compound heterozygous (beta 0/beta +) and results from severe beta globin deficiency. These patients develop severe anemia and bone marrow hyperactivity. Beta-thalassemia major manifests by age 1 to 2 years with symptoms of severe anemia and transfusional and absorptive iron overload. Patients are jaundiced, and leg ulcers and cholelithiasis occur (as in sickle cell disease). Splenomegaly, often massive, is common. Splenic sequestration may develop, accelerating destruction of transfused normal red blood cells. Bone marrow hyperactivity causes thickening of the cranial bones and malar eminences. Long bone involvement predisposes to pathologic fractures and impairs growth, possibly delaying or preventing puberty.

With iron overload, iron deposits in heart muscle may cause heart failure. Hepatic siderosis is typical, leading to functional impairment and cirrhosis. Iron chelation is usually necessary.

Diagnosis of Thalassemias

  • Evaluation for hemolytic anemia if suspected

  • Peripheral smear

  • Hemoglobin electrophoresis

  • DNA testing (prenatal diagnosis)

Thalassemia trait is commonly detected when routine peripheral blood smear and complete blood count show microcytic anemia and elevated red cell count. If desired, the diagnosis of beta thalassemia trait can be confirmed with quantitative hemoglobin studies. No intervention is needed. In females, anemia can be worsened by pregnancy.

More severe thalassemias are suspected in patients with a family history, suggestive symptoms or signs, or microcytic hemolytic anemia. If thalassemias are suspected, laboratory tests for microcytic and hemolytic anemias and quantitative hemoglobin studies (hemoglobin electrophoresis) are done. Serum bilirubin, iron, and ferritin levels are increased.

In alpha-thalassemias, the percentages of Hb F and Hb A2 are generally normal, and the diagnosis of single or double gene defect thalassemias may be carried out with genetic tests. The diagnosis often is one of exclusion of other causes of microcytic anemia.

In beta-thalassemia major, anemia is severe, often with hemoglobin 6 g/dL ( 60 g/L). The red blood cell count is elevated relative to hemoglobin, and the cells are very microcytic. The blood smear is virtually diagnostic, with many nucleated erythroblasts; target cells; small, pale red blood cells; and punctate and diffuse basophilic stippling.

In quantitative hemoglobin studies, mild elevation of Hb A2 only is diagnostic for beta-thalassemia minor. In beta-thalassemia major, Hb F is also usually increased, sometimes to as much as 90%, and Hb A2 is usually elevated to > 3%.

Hb H disease can be diagnosed by demonstrating the fast-migrating Hb H or Bart’s fractions on hemoglobin electrophoresis. The specific molecular defect can be characterized but does not alter the clinical approach.

If bone marrow examination is done for anemia (eg, to exclude other causes), it shows marked erythroid hyperplasia.

Imaging tests done for other reasons in patients with beta-thalassemia major show changes due to chronic bone marrow hyperactivity. The skull may show cortical thinning, widened diploic space, a sun-ray appearance of the trabeculae, and a granular or ground-glass appearance. The long bones may show cortical thinning, marrow space widening, and areas of osteoporosis. The vertebral bodies may have a granular or ground-glass appearance. The phalanges may appear rectangular or biconvex. Chest imaging may reveal evidence of paravertebral extramedullary hematopoiesis.

Prenatal diagnosis and genetic counseling are standard practice for patients with fetuses at risk and can guide fetal therapy in alpha thalassemia major.

Treatment of Thalassemias

  • Often red blood cell transfusion, with or without iron chelation therapy

  • Splenectomy if splenomegaly is present

  • Allogeneic stem cell transplantation if possible

In patients with alpha-thalassemia trait or beta-thalassemia trait, no treatment is needed.

In Hb H disease, splenectomy may be helpful if anemia is severe or splenomegaly is present.

Patients with beta-thalassemia intermedia should receive as few transfusions as possible to avoid iron overload. However, suppression of abnormal hematopoiesis by periodic red blood cell transfusion may be valuable in severely affected patients. In beta thalassemia major, give transfusions as needed to maintain the hemoglobin level around 9 to 10 g/dL (90 to 100 g/L) and avoid severe clinical manifestations.

To prevent or delay complications due to iron overload, excess (transfusional) iron must be removed (eg, via chronic iron chelation therapy). Chelation therapy is generally initiated when serum ferritin levels are > 1000 ng/mL (> 1000 mcg/L) or after about 1 to 2 years of scheduled transfusions. Splenectomy may help decrease transfusion requirements for patients with significant splenomegaly.

1).

Allogeneic stem cell transplantation is the only curative option and should be considered in all patients.

Treatment reference

  1. 1. Cappellini MD, Viprakasat V, Taher A, et al: A phase 3 trial of luspatercept in patients with transfusion-dependent β-thalassemia. N Engl J Med 382(13):1219–1231, 2020. doi: 10.1056/NEJMoa1910182

Prognosis for Thalassemias

Life expectancy is normal for people with beta-thalassemia minor or alpha-thalassemia minor. The prognosis of Hb H disease and beta-thalassemia intermedia varies.

Life expectancy is decreased in people with beta-thalassemia major mostly due to complications from chronic transfusions.

Key Points

  • Thalassemias result from decreased production of at least one globin polypeptide chain (beta, alpha, gamma, delta); the resultant abnormal red blood cells are microcytic, often abnormally shaped, and prone to hemolysis and ineffective erythropoiesis due to damage from the unpaired hemoglobin chains (causing anemia).

  • Splenomegaly, often massive, is common and can result in splenic sequestration that accelerates destruction of red blood cells (including transfused ones).

  • Iron overload is common because of increased absorption (due to defective erythropoiesis) and frequent transfusions.

  • Diagnose using hemoglobin electrophoresis or genetic testing.

  • Transfuse as needed, but monitor for iron overload and use chelation therapy.

  • Splenectomy may help decrease transfusion requirements for patients with splenomegaly.

  • Allogeneic stem cell transplantation is curative.

More Information

The following English-language resource may be useful. Please note that THE MANUAL is not responsible for the content of this resource.

  1. Cooley's Anemia Foundation: provides comprehensive patient education and support and advocacy to patients with thalassemia

quizzes_lightbulb_red
Test your KnowledgeTake a Quiz!
Download the free MSD Manual App iOS ANDROID
Download the free MSD Manual App iOS ANDROID
Download the free MSD Manual App iOS ANDROID